研究成果： |
承担的项目： 国家自然科学基金：嗜热酰基氨肽酶非特异性催化机制的研究及改造 吉林省自然科学基金：高温糖基化生物反应器的构建 国家重点研发计划项目子课题:基于酶和工程菌改造技术的分布式DNA活字存储阵列研究
2017年以来课题组发表文章： [1] Ye Zhou, Jannik Nedergaard Pedersen, Jacob Nedergaard Pedersen, Nykola C. Jones, Søren Vrønning Hoffmann, Steen Vang Petersen, Jan Skov Pedersen, Adam Perriman, Renjun Gao,* and Zheng Guo*，Superanionic Solvent-Free Liquid Enzymes Exhibit Enhanced Structures and Activities, Advanced. Science. 2022, 9, 2202359.
[2] Abidan Ainiwaer, Ao Li, Xingwang Zhao, Yujiao Xu, Siping Han*, and Renjun Gao*, Site-Specific Covalent Immobilization of Methylobacterium extorquens Non-Blue Laccse Melac13220 on Fe3O4 Nanoparticles by Aldehyde Tag, Catalysts. 2022, 12, 1379. [3] Abidan Ainiwaer, Yue Liang, Xiao Ye and Renjun Gao*, Characterization of a Novel Fe2+ Activated Non-Blue Laccase from Methylobacterium extorquens, International Journal of Molecular Sciences. 2022, 23, 9804. [4] Yajing Li, Xue Yang and Renjun Gao*, Thermophilic Inorganic Pyrophosphatase Ton1914 from Thermococcus onnurineus NA1 Removes the Inhibitory Effect of Pyrophosphate, International Journal of Molecular Sciences. 2022, 23, 12735. [5] Fang Wang, Yuchen Liu, Chang Du and Renjun Gao*, Current Strategies for Real-Time Enzyme Activation, Biomolecules, 2022, 12, 599.
[6] Jiabao Lyu, Jian Zhang, Jingxuan Zhu, Siao Chen, Tao Han, Yan Zhang, Renjun Gao, Guiqiu Xie*, Zheng Guo*, Molecular dynamics simulation guided distal mutation of Thermotoga naphthophila β-glucosidase for significantly enhanced synthesis of galactooligosaccharides and expanded product scope,International Journal of Biological Macromolecules, 2022, 210, 21-32. [7] Xiaowen Zhang, Zihao Huang, Dan Wang, Yan Zhang, Bekir Engin Eser, Zhenyu Gu, Rongrong Dai, Renjun Gao*, Zheng Guo*, A new thermophilic extradiol dioxygenase promises biodegradation of catecholic pollutants, Journal of Hazardous Materials, 422 (2022), 126860.
[8] Li Zong, Yan Zhang, Zhengkang Shao, Yingwu Wang, Zheng Guo, Renjun Gao*, Bekir Engin Eser*, Optimization and Engineering of a Self-Sufficient CYP102 Enzyme from Bacillus amyloliquefaciens towards Synthesis of In-Chain Hydroxy Fatty Acids, Catalysts 2021, 11(6), 665. [9] Li Zong, Renjun Gao, Zheng Guo, hengkang Shao, Yingwu Wang*, Bekir EnginEser*, Characterization and modification of two self-sufficient CYP102 family enzymes from Bacillus amyloliquefaciens DSM 7 with distinct regioselectivity towards fatty acid hydroxylation, Biochemical Engineering Journal, 2021, 166, 107871. [10] Jiabao Lyu, Renjun Gao, and Zheng Guo*, Galactosyldiacylglycerols: From a Photosynthesis-Associated Apparatus to Structure-Defined In Vitro Assembling, Journal of Agricultural and Food Chemistry. 2021, 69, 32 [11] Fang Wang, Rong Li, Hui Jian, Zihao Huang, Yingwu Wang*, Zheng Guo*, and Renjun Gao*, Design and construction of an effective expression system with aldehyde tag for site-specific enzyme immobilization，Catalysts, 2020, 10(4), 410. [12] Dongni Liu, Lin Deng, Dan Wang, Wei Li, Renjun Gao*，“Bridge regions” regulate catalysis and protein stability of acylpeptide hydrolase, Biochemical Engineering Journal, 2019, 145, 42-52. [13] Wei Li, Dongni Liu, Xu Geng, Zhengqiang Li* and Renjun Gao*, Real-time regulation of catalysis by remotecontrolled enzyme-conjugated gold nanorod composites for aldol reaction-based applications, Catalysis Science & Technology, 2019, 2221-2230. [14] Ye Zhou, Bianca Pérez, Weiwei Hao, Jiabao Lv, Renjun Gao*, Zheng Guo*, The additive mutational effects from surface charge engineering: A compromise between enzyme activity, thermostability and ionic liquid tolerance, Biochemical Engineering Journal, 2019, 148,195-204. [15] Ye Zhou, Nykola C. Jones, Jannik Nedergaard Pedersen, Bianca Pérez, S. V. Hoffmann, Steen Vang Petersen, Jan Skov Pedersen, Adam Perriman, Peter Kristensen, Renjun Gao*, and Zheng Guo*, Insight into the Structure and Activity of Surface-Engineered Lipase Biofluids, ChemBioChem, 2019, 20, 1266-1272. [16] Jiabao Lyu, Zidan Li, Juncheng Men, Ruyun Jiang, Guilin Tang, Ye Zhou, Renjun Gao*, Covalent immobilization of Bacillus subtilis lipase A on Fe3O4 nanoparticles by aldehyde tag: An ideal immobilization with minimal chemical modification, Process Biochemistry, 2019, 81, 63-69. [17] Dongni Liu, Wei Li, Xiaoyu Jiang, Shuju Bai, Jiyang Liu, Xutong Liu, Yuhua Shi, Ziyu Kuai, Wei Kong, Renjun Gao* and Yaming Shan*, Using near-infrared enhanced thermozyme and scFv dual-conjugated Au nanorods for detection and targeted photothermal treatment of Alzheimer’s disease, Theranostics, 2019, 9(8), 2268-2281. [18] Jingwen Yang, Renjun Gao, Ye Zhou, Sampson Anankanbil, Jingbo Li, Guiqiu Xie*, Zheng Guo*, β-Glucosidase from Thermotoga naphthophila RKU-10 for exclusive synthesis of galactotrisaccharides: Kinetics and thermodynamics insight into reaction mechanism, Food Chemistry, 2018, 240, 1:422-429 [19] Jingwen Yang, Bianca Pérez, Sampson Anankanbil, Jingbo Li, Ye Zhou, Renjun Gao*, Zheng Guo*, Valorizing Dairy Waste: Thermophilic Biosynthesis of a Novel Ascorbic Acid Derivative, Journal of Agricultural and Food Chemistry, 2017, 65(41): 9087-9093. [20] Jingwen Yang, Qi Wang, Ye Zhou, Jingbo Li, Renjun Gao*, Zheng Guo*, Engineering T. naphthophila β-glucosidase for enhanced synthesis of galactooligosaccharides by site-directed mutagenesis, Biochemical Engineering Journal, 2017, 127, 15:1-8. [21] Jingwen Yang,Bianca Pérez, Sampson Anankanbil,Jingbo Li, Renjun Gao* and Zheng Guo*, Enhanced Synthesis of Alkyl Galactopyranoside by Thermotoga naphthophila β-Galactosidase Catalyzed Transglycosylation: Kinetic Insight of a Functionalized Ionic Liquid-Mediated System, ACS Sustainable Chem. Eng. 2017, 5, 2006-2014. [22] Ye Zhou, Guiqiu Xie, Lin Chang, Yan Wang, Renjun Gao*, Identification of an archaeal maltooligosyltrehalose trehalohydrolase encoded by an interrupted gene, Extremophile, 2017, 21:537-549.
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